SynTox 3D toxicology model provides real-time optical monitoring, multi-compartment, multi-cellular architecture, and low reagent requirements. Other benefits of this platform are:
- Physiologically realistic morphological, fluidic and 3D cellular conditions
- Universal platform with architecture-specific of the desired organ
- Significant reduction in cost and time
- Robust and easy to use protocols
- Compatible with standard analytical instruments for both on-chip and off-chip assays including omic methodologies for systems biology and bioinformatic analysis
SynTox 3D Toxicology Model recreates the in vivo micro-environment by modeling a histological tissue slice in an in vitro environment.
Current in vitro platforms are poor predictors of in vivo safety, efficacy, and pharmacokinetics of therapeutics owing to the significant differences in the test conditions compared to physiological conditions observed in vivo.
Current in vitro models routinely utilize 2D monolayers or 3D aggregates of cells under static conditions for studying drug toxicity. These models fail to reproduce in vivo physiological features such as morphological size, physiological blood flow, and cellular (biological) make-up of the specific organs being investigated. Other microfluidic models employ a membrane-based top-bottom two-compartment architecture, inherently limiting key desired features such as real-time visualization and the ability to simultaneously analyze multi-cellular cultures.
Examples of Models Developed Using SynTox Devices
Liver model with endothelial cells and hepatocytes. Acetaminophen toxicity on hepatocytes following bolus injection. Peripheral hepatocytes show severe toxicity.
Drug toxicity on Cardiac cells. Left panel indicates viable cells while right panel indicates mixture of live and dead cells following drug treatment.
PRODUCT PURCHASING OPTIONS
Chips: Depending on your specific research applications you can select from IMN2 radial or linear co-culture chip configurations.
Kits: All the basic components required to run SynTox assays can be purchased in a kit format. Two Kit formats are available.
Starter Kit: Select this for your first-time purchase
- 10 SynTox chips (Choice of IMN2 radial or linear chips)
- Accessories including tubing, clamps, needles, and syringes
- Pneumatic priming device (required for priming tubing to remove air)
Assay Kit: Select this kit format if you have previously purchased the pneumatic priming device
- 10 SynTox chips (Choice of IMN2 radial or linear chips)
- Accessories including tubing, clamps, needles, and syringes
Idealized Co-Culture Network Chips (IMN2 radial)
Idealized Co-Culture Network (IMN2 Radial) Chips: 2 um slits. 50um Travel (distance between channels), 100um Depth (height). Cat# 102016
Starter Kit
$1,700Add to cart
Assay Kit
$1,500Add to cart
Idealized Co-Culture Network Chips (IMN2 Linear)
Idealized Co-Culture Network (IMN2 Linear) Chips: 3 um slits. 50um Travel (distance between channels), 100um Depth (height). Cat# 108013
Starter Kit
$1,700Add to cart
Assay Kit
$1,500Add to cart
SynTox Liver model was used to demonstrate the toxicity of classical pain killer acetaminophen using different modes of interaction.
Hepatocytes were cultured in the SynTox model and analyzed for functionality using standard commercially available assays. Hepatocytes formed bile-canaliculi, produced urea in increasing concentration in a time-dependent manner with upregulated enzyme activity under physiological fluid flow conditions.
Hepatocytes form bile-canaliculi (Figure A), secrete urea with increased production in a time-dependent manner (Figure B) and can be monitored in real-time for enzyme activity (Figure C). The effect of flow on hepatocytes is clearly observed with increased enzyme activity.
SynTox Toxicology Model was used for the understanding of drug toxicity responses using systems biology-based analysis.
Liver cells and heart cells were co-cultured with their respective endothelial cells in the SynTox microfluidic chip and treated with Doxorubicin. The cells were harvested and subjected to genomic analysis resulting in upregulated and downregulated genes. The identified genes were used to develop systems biology-based cellular pathway model for the identification of targets and mechanisms.
Genomic responses highlighting upregulated and down regulated cells in Endothelial Cells (Figure A), Hepatocytes (Figure B) and Cardiac Cells (Figure C)
Mono cultured hepatocytes or co-cultured with endothelial cells were treated with acetaminophen and their drug responses were investigated for different modes of interactions using a combination of Live/Dead and Reactive Oxygen Species (ROS) assays. Differences between vascular and direct treatment of hepatocytes was observed following drug interaction.
Real-time Monitoring of Drug Diffusion and Drug Toxicity
SynTox microfluidic chip can be used to investigate drug diffusion in real-time across the vascular endothelium and the tissue cells to determine the duration for minimum or maximum exposure to the cells. The information obtained can be used to predict time-dependent toxicity.
Real-time monitoring of drug diffusion and time-dependent toxicity. Drug diffusion across the endothelium-tissue interface (Figure A). Time-dependent increase in apoptotic cells following chemotherapeutic treatment (Figure B). Time-dependent increase in ROS intensity indicative of toxicity following chemotherapeutic treatment (Figure C).
Assay Development and Screening using SynTox:
Models Available:
- Monoculture using endothelial cells
- Co-Culture with stromal/tissue cells
Assays:
- Drug-induced vascular leakage
- Vascular inflammation
- Biomarker analysis
- Efficacy and toxicity screening
Endpoints to choose from:
Vascular permeability using fluorescent-tagged molecule, Viability, ROS, biomarkers, collect cells or effluents for downstream genomic, proteomic or metabolomic analysis.
SynTox 3D Model – Starter Kits
Includes consumables (10 chips, 100ft tubing, 25 slide clamps, 50 blunt tip needles, and 50 1 ml syringes). Starter kits will also include the pneumatic priming device (required for running SynTox assays).
Note: Does not include other required consumables such as cells, media, and matrix. Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.
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CAT# | Product Name | Price: |
|---|---|---|
404002 | ||
404004 |
SynTox 3D Model – Assay Kits
Includes consumables (10 chips, 100ft tubing, 25 slide clamps, 50 blunt tip needles, and 50 1 ml syringes).
Note: Does not include other required consumables such as cells, media, and matrix. Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.
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CAT# | Product Name | Price |
|---|---|---|
404001 | ||
404003 |
SynTox 3D Model – Chips
Purchase single chips.
Note: Does not include other required consumables such as cells, media, and matrix. Laboratory equipment required includes incubators, inverted microscopes, and syringe pumps.
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CAT# | Product Name | Price |
|---|---|---|
102016-STo3 | ||
108013-STo3 |